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Clone, Expression and Immunological Analysis of Neisseria meningitidis Outer Membrane Protein NhhA |
CHEN Liu,LI Jian-feng,XU Wei-ming,DAI Zong-xiang,LI Zhi-hua,SUN Qiang-ming |
Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming 650118, China |
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Abstract Neisseria meningitidis gene GNA0992 was cloned into the prokaryotic expression vector pET-20b, and soluble expressed in E.coli BL21 (DE3). Purified NhhA stimulated a significant antigen-specific humoral response following three intraperitoneal immunization, while bactericidal assay showed that NhhA can elicit antibodies capable of inducing bactericidal activity against group B N.meningitidis strains. The results indicate that NhhA is a good candidate for vaccine development.
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Received: 18 November 2009
Published: 29 April 2010
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