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Effects of Chitosan Oligosaccharides Attenuating Menadione-induced Injury in Macrophages |
MA Pan1,2, LIU Hong-tao1,2, XU Qing-song1, BAI Xue-fang1, DU Yu-guang1 |
1. Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China;
2. Graduate School of Chinese Academy of Sciences, Beijing 100049, China |
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Abstract Aim: To investigate the effects of Chitosan oligosaccharides (COS) on attenuating menadione-induced stress injury in macrophages. Method: MTT assay was used to examine the cell viability, and the reagent kits were used to investigate the activities of enzymes and the products of lipid peroxidation in redox system. Results: By using MTT assay and detecting the activities of LDH (lactate dehydrogenase) in the cultural supernatants, it was confirmed that chitosan oligosaccharides could attenuate menadione-induced stress injury in macrophages. Moreover, the results showed that COS could also restore activities of endogenous antioxidants including superoxide dismutase (SOD) and glutathione peroxidase (GSH-PX), and suppressing the production of lipid peroxidation. Those results could explain the mechanisms of COS on attenuating menadione-induced stress injury in macrophages. Conclusion: Stress injury induced by menadione in macrophages could be blocked by COS.
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Received: 20 December 2010
Published: 28 June 2011
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