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Adult Stem Cells Harvested by Implanting Gelatin Sponges into Spatium Intermusculare |
WU Xiao-yun1, WANG Shi-li1, MU Zhong-xiang2 |
1. Key Laboratory for Biotech-Drugs Ministry of Health Shandong Medicinal Biotechnology Center, Jinan 250062, China;
2. Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan 250011, China |
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Abstract A new method to harvest stem cells was established by implanting biomaterials into mice. Gelatin Sponges (GS) were implanted into the spatium intermusculare of mice hind limbs. A large number of migrating cells were isolated from the transplanted biomaterials at 12 days after implanting. The characteristics of adherent cells were similar to that of bone marrow (BM) stem cells, including morphology, proliferation potential and multilineage differentiation capacity. The frequency of CFU-F in GS (82.2±10.6/105) was much higher than in BM (43.7±7.4/106) (P<0.05). In additional, BM transplantation demonstrated that stem cells in the GS originated from the PB. Moreover, it is possible for autologous cell therapy, so this method may be a promising alternative for the clinical application.
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Received: 23 November 2010
Published: 28 June 2011
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