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中国生物工程杂志

China Biotechnology
China Biotechnology  2011, Vol. 31 Issue (06): 116-123    DOI:
    
Advances on the Study of Multi-gene Expression System
CHU Su-xia1,2, YAO Lun-guang2, XING Yan-hao1, ZHOU Yan-qing1
1. School of Life Sciences, He Nan Normal University, Xinxiang 453007,China;
2. He Nan Provincial Key Laboratory of Funiu Mountain Insect Biology, China-UK, Nan Yang Normal University-Rothamsted Research Joint Laboratory of Insect Biology, Nanyang Normal University, Nan yang 473061, China
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Abstract  

Most of cellular proteins work as the subunits of protein complexes which are the basic functional unit in cell. The structure research and functional analysis of macromolecule complexes became popular in the post-genomic era. One major challenge is to produce these complexes in sufficient amounts for biochemical and structural research. One efficient way which named mutigene expression system is used widely to obtain the protein complexes by co-expressing subunits simultaneously in host cells. Multigene expression system is becoming more and more important on both basic and application researches. The studies on multigene expression system have been developed quickly, especially in the recent five years. The studies on multigene expression system, which provided the knowledge on it's characters and usages were summarized. The detailed construction methods of different kinds of multigene expression vectors, especially the newest vectors, which will be helpful to choose the most efficient system for protein complexes studies were introduced. The primary applications of the multigene expression system are also introduced.



Key wordsProtein complex      Multigene expression system      Expression cassette      Homing endonuclease      Isocaudamer technique     
Received: 21 December 2010      Published: 28 June 2011
ZTFLH:  Q786  
Corresponding Authors: lunguang Yao     E-mail: lunguangyao@163.com
Cite this article:

CHU Su-xia, YAO Lun-guang, XING Yan-hao, ZHOU Yan-qing. Advances on the Study of Multi-gene Expression System. China Biotechnology, 2011, 31(06): 116-123.

URL:

https://manu60.magtech.com.cn/biotech/     OR     https://manu60.magtech.com.cn/biotech/Y2011/V31/I06/116

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