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| Cloning and Expression Characteristic of CSEBF1 Gene in Cucumber (Cucumis stavius L.) |
| JIN Xiao-xia1, DONG Yan-long2, QIN Zhi-wei3, YU Li-jie1, WU Shu-ju1, SONG Jian1 |
1. College of Life Science and Technology, Harbin Normal University, Harbin 150025, China;
2. Horticulture branch, Heilongjiang academy of agricultural sciences, Harbin 150060, China;
3. College of horticulture, Northeast Agriculture University, Harbin 150030, China |
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Abstract Based on selecting SSH of stem tips treated by ethrel, the cDNA sequence of cucumber EIN3-Binding F box protein 1 gene, named CSEBF1(GenBank: KF366911) was obtained by RT-PCR and in silico cloning. The cDNA sequence of the CSEBF1 gene was 1 964bp, contained a predicted protein sequence of 640 amino acids, the F-box sequences and essential structure for ubiquitin-mediated proteolysis—LRRs (leucine-rich repeats). The predicted amino acids sequence of cucumber CSEBF1 shared 60.47% identity with Arabidopsis. The mRNA expressions of CSEBF1 in different tissues treated by ethrel were detected through real-time quantitative RT-PCR, and results revealed that highest level of CSEBF1 mRNA was increased by ethrel at 8h in leaf and root, and at 16h in stem. Meanwhile, the expressions of genes involved in ethylene signal transduction were detected through RT-PCR, revealed that CSEIN3 expression was induced in stem and leaf at 4 h; the mRNA accumulation of CSCTR1 was detected in stem at 8 h and leaf at 16 h; the expression of CSACS2 was induced in leaf at 2 h, and increased subsequently. The mRNA of CSACS1G was increased in leaf at 4 h, and then continued to lower levels.
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Received: 20 December 2013
Published: 25 March 2014
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