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Clone and Sequence Analysis of Antheraea pernyi Nucleopolyhedrovirus PstⅠ-B and C fragments |
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Abstract Antheraea pernyi nucleopolyhedrovirus (ApNPV) PstⅠ-B and C fragments were cloned and sequenced. ApNPV PstⅠ-B was 7406 bp long, contained seven open reading frames (orfs)/genes, including p87, he65, pnk/pnl, odv-ec43 and Orgyia pseudotsugata multicapsid nucleopolyhedrovirus (OpMNPV) orf107, orf108 homologue, on either strands of genomic DNA. ApNPV PstⅠ-C was 6663 bp long, contained eleven orfs/genes, including pk-1, orf1629, polh, lef-2, ptp-2, ctl-1, ptp-1 and OpMNPV orf5, orf7, orf8, orf11 homologue, on either strands of genomic DNA. ApNPV was the third baculovirus found contain pnk/pnl gene, the fourth baculovirus found contain both ptp-1 and ptp-2 gene.
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Received: 11 September 2006
Published: 10 May 2010
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