中国生物工程杂志

To construct a oligonucleotide microarray for the detection of five important pathogens including CMV、RV、HSVI、TB、EBV in central nervous system..METHODS : 70 mer oligo probes targeted the specific protein of 5 common pathogens of infectious diseases of the nervous system were designed for the fabrication of microarray. DNA was extracted with conmercilized kit. The target genes were labeled with biotin-dUTP by random primer extension method using the DNA as template. The labeled products were hybridized with the oligonucleotide microarray. After hubridization the microarray were binding with avidin-CY3. After scan with Scanarray 4000 confocal scanner, the fluorescent intensity was analyzed with Quantarray3.0 software. This oligonucelotide microarray system were used to detect clinical sample and compared with PCR method. RESULT: The specific probes for the pathogens are very specific for the detecting the presence of the pathogens. Clinical detection results showed the specificity and sensitivity of this microarray system are 95% and 83% respectively. There is no significant difference between our method and the routine PCR method.. CONCLUSION:The microaaray system for the detection of the major pathogen of central nervous system has the advantage of specific,sensitive and parallel detection. We can add pathogen probes according to the practical needs. It can not only be used for the diagnosis of central nervous system, but also canbe used for the detection of blood, urine, and other body fluid infection.