Expression of Plant Monellin in Escherichia coli Induced by Lactose

China Biotechnology

2008, Vol. 28

Issue (3): 53-58 DOI:

Expression of Plant Monellin in Escherichia coli Induced by Lactose

Download:

HTML

PDF(810KB) HTML
Export: BibTeX | EndNote (RIS)

Abstract

According to the amino acid sequence of monellin and the bias in coden choice in Escheria coli，a single chain monellin gene was synthesized and subcloned into a Escheria coli expression vector pET-28a. The recombined plasmid pET-28a-Mon was then transformed into Escheria coli BL21(DE3).Lactose was used as an inducer instead of IPTG.Through proper optimization of induction conditions，an expression level 33.09% of total cellular protein was achieved.The expression level of recombinant protein induced by lactose was basically the same as that induced by IPTG.The result indicated that lactose could be used an promising inducer in the production of recombined Monellin.

Key words： monellin lactose inducing expression optimization

Received: 07 September 2007 Published: 28 March 2008

	Service
	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors

Cite this article:

. Expression of Plant Monellin in Escherichia coli Induced by Lactose. China Biotechnology, 2008, 28(3): 53-58.

URL:

https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2008/V28/I3/53

[1]	WANG Xiao-jie,MENG Fan-qiang,ZHOU Li-bang,LV Feng-xia,BIE Xiao-mei,ZHAO Hai-zhen,LU Zhao-xin. Breeding of Brevibacillin Producing Strain by Genome Shuffling and Optimization of Culture Conditions[J]. China Biotechnology, 2021, 41(8): 42-51.

[2]	JIANG Ji-zhe, PAN Hang, YUE Min, ZHANG Le. *The Study of Worldwide Brucella canis* of Phylogenetic Groups by Comparative Genomics-based Approaches**[J]. China Biotechnology, 2020, 40(3): 38-47.

[3]	Xin-miao WANG,Kang ZHANG,Sheng CHEN,Jing WU. Recombinant Expression and Fermentation Optimization of Dictyoglomus thermophilum Cellobiose 2-Epimerase in Bacillus subtilis[J]. China Biotechnology, 2019, 39(7): 24-31.

[4]	Long-bing YANG,Guo GUO,Hui-ling MA,Yan LI,Xin-yu ZHAO,Pei-pei SU,Yon ZHANG. Optimization of Prokaryotic Expression Conditions and Antifungal Activity Detection of Antibacterial Peptide AMPs17 Protein in Musca domestica[J]. China Biotechnology, 2019, 39(4): 24-31.

[5]	Yue WANG,Jiang-hua LI,Guo-cheng DU,Long LIU. Molecular Modification of L-amino Acid Deaminase and Optimization of α-ketoglutaric Acid Production by Whole-cell Biocatalysis[J]. China Biotechnology, 2019, 39(3): 56-64.

[6]	REN Li-qiong,WU Jing,CHEN Sheng. Co-Expression of N-Acetyltransferase Enhances the Expression of Aspergillus nidulans α-Glucosidase in Pichia pastoris[J]. China Biotechnology, 2019, 39(10): 75-81.

[7]	Yi-ying WANG,Hai-rong CHENG. *Cell Surface-Displaying the Lactose Hydrolase on Yarrowia lipolytica: a New Approach to Lactose Hydrolysis*[J]. China Biotechnology, 2018, 38(8): 41-49.

[8]	Hai-jiao JI,Wen-lei LI,Rui-jing Huang,Jian LI,Han-mei XU. Anti-CD20rh MAb Quality Evaluation and Monoclonal Cell Line Screening[J]. China Biotechnology, 2018, 38(8): 34-40.

[9]	Ya-li HAN,Guang-heng YANG,Yan-wen CHEN,Xiu-li GONG,Jing-zhi ZHANG. The Optimization of Self-deleting Lentiviral Vector Carrying Human β-globin Gene and Promoter[J]. China Biotechnology, 2018, 38(7): 50-57.

[10]	Li ZHANG,Juan DING,Yu-cheng HAO,Cheng YE,Yang PU. Identification of a Marine Microalgae and Optimization of Protoplast Preparation[J]. China Biotechnology, 2018, 38(11): 42-50.

[11]	Jing WANG,Xin XU,Xue-yu WANG,Lun-guang YAO,Yun-chao KAN,Jun JI. Research Progress of Loop-Mediated Isothermal Amplification in Food Safety Testing[J]. China Biotechnology, 2018, 38(11): 84-91.

[12]	FENG Xue, GAO Xiang, NIU Chun-qing, LIU Yan. *Construction of Pichia pastoris* Expression Vector of Codon Optimized αB-crystallin Gene and Expression Optimization**[J]. China Biotechnology, 2017, 37(7): 42-47.

[13]	ZHANG Xu-hui, ZHANG Hong-nan, LI Yong, WANG Wen-qiang. *Screening and Identification of Biocontrol Fungi against Didymella bryoniae* and Optimization of Fermentation Conditions**[J]. China Biotechnology, 2017, 37(5): 76-86.

[14]	YAO Ren-hui, DONG Zhuo, LI Hui. *Biotransformation of Androst-4-en-3,17-dione by Gibberella intermedia* C2**[J]. China Biotechnology, 2017, 37(3): 73-77.

[15]	LIN You-hong, CHENG Xia-ying, YAN Yi-wen, LIANG Zong-suo, YANG Zong-qi. *Expression and Optimization Strategy of Recombinant Proteins in Chlamydomonas* Chloroplast**[J]. China Biotechnology, 2017, 37(10): 118-125.

Viewed

Full text

Abstract

Cited

Shared

Discussed