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Studies of Temperature Shift Fermentation for Eicosapentaenoic Acid Production by Nitzschia laevis |
didi422@163.com |
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Abstract Fermentation for Eicosapentaenoic Acid (EPA) production by Nitzschia laevis at various temperature between 10℃ and 30℃ was investigated and the dynamics characteristics during fermentation process were also analyzed. Based on the results, a varying temperature nursing method of two stage control strategy is proposed: During the firs stage, which comprises the delay phase and the initial index phase, the temperature is maintained at 25℃; then the temperature is shifted to 20℃ and kept up till the end of the fermentation process. By this method, a EPA content of 6.0% and a yield of 291.60 mg·L-1 have been gained. These are 24.07% and 18.81% higher than that of fixed temperature (25℃) fermentation, respectively.
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Received: 09 August 2007
Published: 25 December 2007
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