中国生物工程杂志

HBeAg is an ungranular secretory protein, which encoded by C gene of HBV DNA and it increases with the replication of HBV. So it is one of the markers of active replication of HBV in clinical diagnosis. HBeAg is important biologic raw materials which is widely used in preparation of related diagnostic articles with HBV infection serological detection. The technology of expression and purification of recombinant HBeAg is quite mature, which had successfully expressed the target protein in various expression systems. The key factors on HBeAg expression include important site mutation in precore region ,the choice of vectors, effects of RNA interference（RNAi）and so on. Therefore, in order to meet requirements of related diagnostic products, it need to improve expression level and purity of recombinant HBeAg and avoid cross-reaction with HBcAg. In a word, it showed that acquisition of high quality recombinant HBeAg could lay substantial foundation for improving diagnostic products, provide a reliable evidence for exploiting new type of theapical and preventive HBV vaccine and offer possibility of HBeAb detection methodological optimization.