Abstract For the important role in economic production, the study has been focused on the technique of cryopreservation of boar semen. During earlier time the study mainly focused on screening and optimizing the cryprotective mediums and freezing methods, which exploited the technique of cryopreservation deeply. However, the study indicated that the fecundation ability was decreased no matter what cryoprotective mediums and what freezing methods were used, companied with the losing of proteins, changing of proteins expressing quantity and increasing or decreasing of the function and activity in sperm after cryopreservation. Now, the study is altering to exploit the essence of the change of protein structure and function, and elucidate the cryodamage mechanism of sperm cryopreservation, which may provide the basic theory for cryopreservation of boar semen and promote the rapid development of the technique of boar semen cryopreservation.
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