Studies on the extraction and toxicity of exopolysaccharide from Rhizobium sp. N613

China Biotechnology

2008, Vol. 28

Issue (3): 74-78 DOI:

Studies on the extraction and toxicity of exopolysaccharide from Rhizobium sp. N613

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Abstract

The process of extracting exopolysaccharide(REPS) from Rhizobium sp. N613 was optimized in this study. The optimized conditions of extracting REPS were obtained by Response Surface Methodology. They are pH value 5.9, concentration of ethanol 74% and precipitation time 16.5 h, respectively. Under those conditions, the extraction yield was 9.28±0.06 g · L -1, the purity was up to 97% and the extraction rate was 93.6%. At the same time, the thesis carried out the test of the viscosity、 acute toxicity and accumulative toxicity of REPS. It is showed that REPS has high viscosity and nontoxicity. So it is more valuable for the application for food and medicine.

Key words： exopolysaccharide Response Surface Methodology extraction acute toxicity accumulative toxicity

Received: 28 November 2007 Published: 28 March 2008

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Cite this article:

. Studies on the extraction and toxicity of exopolysaccharide from Rhizobium sp. N613. China Biotechnology, 2008, 28(3): 74-78.

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https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2008/V28/I3/74

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