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中国生物工程杂志

China Biotechnology
China Biotechnology  2010, Vol. 30 Issue (04): 13-19    DOI: Q343.6
    
Enhanced Production of Human Nerve Growth Factor by CHO Cells Through the Control of Culture Conditions
State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071,China
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Abstract  

The enhancement of recombinant protein productivity of a stable cell line is essential for developing an efficient large-scale bioprocess. The effect of some media additives and temperature conditions were studied in an attempt to optimize cell growth and protein productivity from a Chinese hamster ovary (CHO) cell line producing human nerve growth factor. The addition of dimethyl sulfoxide (DMSO), sodium butyrate (NaB), KH2PO4 and temperature shift (37℃ and 32℃) were evaluated for the enhancement of hNGF productivity in a fed batch suspension culture. The samples were taken everyday for checking their viable cell density, viability, hNGF productivity and glucose concentration. The culture time was prolonged significantly from 7 days to 14 days when the culture temperature decreased from 37℃ to 32℃. The productivity of hNGF increased in addition of NaB and DMSO though they arrested the cell cycle dramatically. The maximum specific hNGF productivity was achieved in the medium with 2% DMSO cultured at 37℃ and the maximum hNGF productivity was achieved in the medium with 3.65mmol/L KH2PO4 cultured at 32℃. Taken together, the addition of 3.65mmol/L KH2PO4 or 1% DMSO in the culture medium had the greatest effect on hNGF productivity in CHO cells.



Key wordsCHO cells      Sodium butyrate      DMSO      KH2PO4     
Received: 14 December 2009      Published: 29 April 2010
Corresponding Authors: Hou Li-Hua     E-mail: chenwei0226@yahoo.com.cn;houlihua@sina.com
Cite this article:

SONG Xiao-Gong, XU Ting, LI Bing, FU Ling, HOU Li-Hua, CHEN Wei. Enhanced Production of Human Nerve Growth Factor by CHO Cells Through the Control of Culture Conditions. China Biotechnology, 2010, 30(04): 13-19.

URL:

https://manu60.magtech.com.cn/biotech/Q343.6     OR     https://manu60.magtech.com.cn/biotech/Y2010/V30/I04/13

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