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| Expression of Chitinase Gene of HaSNPV, and Purification,Refolding of Its Recombinant Protein |
| College of Chemical Engineering, Northwest University, Xi’an 710069,China |
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Abstract Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus (HaSNPV) has been extensively used for many years to control H.armigera in China. HaSNPV chitinase is considered responsible for the liquefaction process of cadavers, and it may be a safer pesticide to control H.armigera.The HaSNPV chitinase gene was constructed into pET28a and transferred into E.coli strain Rosetta, which contain colons rarely used in E.coli. The chitinase was successfully expressed as (His) 6-chiA. It was expressed as inclusion body and was purified by Ni-NTA Column Affinity Chromatography under denaturing conditions. The inclusion body was then refolded by two different ways, both of which can produce activated enzyme.
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Received: 21 September 2009
Published: 26 February 2010
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