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| Development of a Reverse Dot Blot System for rapid detection of HBV Genotypes |
| ZHAO Li,ZHANG Wen-lu,HU Yuan,LIU Yan-chen,LAI Guo-qi,YANG Feng,HUANG Ai-long |
| Key Laboratory of Molecular Biology on Infectious Diseases，Ministry of Education， Chongqing Medical University ，Chongqing 400016，China |
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Abstract To develop a reverse dot blot assay for rapid detection of HBV genotypes. Specific oligonucleotides probes were desighed and immobilized on nylon membranes. The DNA sample to be tested was PCR-amplified with DIG labeling primers and then hybridized with the immobilized probes. This procedure for detecting HBV genotypes was simple,rapid and specificity. 30 specimens in Chongqing area were collected and detected by this method, and results were evaluated using direct sequencing. Results showed that: This new method was applicable to precise detection HBV genotypes for specimen with copies up to 103, and the HBV genotyping results showed that genotype B was the predominant genotype in Chongqing area．
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Received: 28 July 2009
Published: 21 December 2009
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