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| Research on HIV Pseudovirion as Positive Control for p24 Antigen Detecting Kit |
| ZHAO He-long,ZHAO Peng-xiang,LI Zheng,MA Xue-mei ,ZHONG Ru-gang,ZENG Yi |
| College of Life Science and Bioengineering, Beijing University of Technology, Beijing 100124, China |
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Abstract A new kind of p24 antigen positive control was developed to provide a better solution to the problems encountered in developing a novel HIV detecting kit, such as (a) inconveniency in storing p24 positive control, (b) difficulty in maintaining p24 antigen activity, (c) differences in protein structure and aggregation mode between recombinant p24 antigen and natural p24 antigen. Modified HIV1 skeleton plasmid pNL43 EGFP R-E-and envelope protein plasmid pGX-C were co-transfected into 293T cell, and then culture fluid containing single-round HIV pseudovirions were collected. This kind of HIV pseudovirions possess natural core shell protein p24, which could be used as the positive control for detection of HIV p24 antigen. Through comparison of activity and stability between pseudovirion p24 antigen and positive control in common detecting kit, it was proved that pseudovirion p24 is more convenient to store, more stable and more similar to natural p24 protein structure and aggregation mode than common recombinant p24 protein.
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Received: 16 March 2010
Published: 25 August 2010
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Corresponding Authors:
Ma Xue-Mei
E-mail: xmma@bjut.edu.cn
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