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| Expression of NSP1 of a Group A Human Rotavirus and Immunological Properties |
| Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming 650118, China |
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Abstract The non structural protein 1 (NSP1) of rotavirus (RV) plays a very important role in interaction between virus and host. In this article, the full-length gene of the NSP1 of strain TB-Chen was expressed in E.coli BL21(DE3) cells by using gene cloning and expression technologies, and immunogenicity, synthesis and subcellular distribution of the NSP1 protein in RV infected cells, and phylogenesis and genotypes of the NSP1 were studied. The results showed that the recombinant NSP1 protein (rNSP1) could be highly expressed in E.coli BL21(DE3) cells, which possesses 34.4% of total cell proteins. The rNSP1 could elicit specific antibodies in guinea pigs, the antibodies could react with the rNSP1 itself and NSP1 protein expressed in SA11 infected or Wa infected MA104 cells by Western blot and immunofluoresence assay. The results of immunofluoresence also showed that NSP1 protein was aggregated in the cytoplasm and formed radiate granular structure in SA11 infected MA104 cells but not in Wa infected MA104 cells. At least 16 recognized genotypes of NSP1 were classified in group A RVs, based on diversity of sequences of the NSP1 open reading frame, and TB-Chen strain NSP1 was defined as genotype A2. RVs with different NSP1 genotype have specific host ranges, RVs possessing the same NSP1 genotype can infect different species, the same species can be infected with RVs possessing different NSP1 genotypes. Genotypes A4 and A16 were only found in birds and only A4 and A16 were found in birds. The results obtained in the present study are important for further studying on structure, function and immunological properties of the NSP1.
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Received: 27 September 2009
Published: 25 March 2010
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