Evaluation of the Immunity Effectiveness on the Engineered Subunit Vaccine of Staphylococcus aureus α-Hemolysin

China Biotechnology

2009, Vol. 29

Issue (11): 17-22 DOI:

Evaluation of the Immunity Effectiveness on the Engineered Subunit Vaccine of Staphylococcus aureus α-Hemolysin

ZHANG Hai-yan^1,2,YANG Hong-jun¹,WANG Chang-fa¹,HE Hong-bin¹,ZHONG Ji-feng¹,YANG Shao-hua¹,MA Wei-ming²

1.Cow Research Center ,Agricultural Science Academy of Shandong Province, Jinan 250100,China
2.College of Veterinary Medicine，Shandong Agricultural University, Taian 271018,China

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Abstract

Objective:α -Hemolysin of Staphylococcus aureus, which was expressed in E.coli BL21(DE3) with recombinant pET32a+-α-HL plasmid, was purified with gel filtration chromatography(GFC), and then the engineered subunit vaccine was developed. The immunity effectiveness of this vaccine was evaluated on mouse models.Method:The purified fusion protein was analyzed in SDS-PAGE, and subjected to the evaluation of its median hemolytic dose potency(HD50) was finally analyzed with rabbit erythrocyte. Protein concentration was determined by the method of Bradford. Antibody titers were evaluated on ELISA, and then challenged to gain the immunity protect index.Results:There is an expected protein band with molecular mass of 53kDa in SDS-PAGE, and the concentration is 0.1278mg/ml. The hemolysis activity is 8012.5 HU/mg. There are specific antibodies acquired in bloodserum from mice after vaccined and the antibody titers rising until it has arrive the max, then following down.Conclusion:The purified fusion protein has good fineness and hemolysis activity, the antibody titers initiated by the protein vaccine go with regulation and the immunoprotection is satisfied.

Key words： Staphylococcus aureus a-hemolysin Engineered subunit vaccine Immunity effectiveness

Received: 02 April 2009 Published: 07 December 2009

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	Articles by authors
	ZHANG Hai-Yan
	YANG Hong-Jun
	WANG Chang-Fa
	HE Hong-Ban
	ZHONG Ji-Feng
	YANG Shao-Hua
	MA Wei-Meng

Cite this article:

ZHANG Hai-Yan, YANG Hong-Jun, WANG Chang-Fa, HE Hong-Ban, ZHONG Ji-Feng, YANG Shao-Hua, MA Wei-Meng. Evaluation of the Immunity Effectiveness on the Engineered Subunit Vaccine of Staphylococcus aureus α-Hemolysin. China Biotechnology, 2009, 29(11): 17-22.

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https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2009/V29/I11/17


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