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| Construction of RNAi Expression Vector by Fusion Gene Fragments of BBE and COR from Opium Poppy |
| LIANG Qian-qian1,3,ZHANG Jin-wen1,WEI Yu-jie2,WANG Wang-tian1,HE Qing-xiang2,LEI Yao-hu2 |
1.College of Agronomy, Gansu Agricultural University, Lanzhou 730070,China
2.Agricultural Research Academy of Gansu Reclamation, Wuwei 733006,China
3.Hexi University, Zhangye 734000,China |
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Abstract Abstract: The codeinone reductase gene and the berberine bridge enzyme gene were cloned from young leaf of opium poppy(Papaver somniferum L) by RT- PCR and the coding sequences of these gene were analyzed. The result demonstrated that the cloned COR gene sequences were analyzed to be highly homologous with the COR gene family members and exhibited homologous of 98.96% with COR1.1.The cloned BBE gene sequences were 94.84% identified with the reported BBE genes in GenBank previously. Based on the cDNA sequences of COR and BBE ,two fragments about 400～500 bp from each gene with lower identity among them were cloned. The fusion gene BC (744 bp) is fused by the PCR technique. Then the promoter CaMV 35S driven, containing‘forward BC fusion fragments-reverse pdk intron-reverse BC fusion fragments’, plant siRNA expression vector were constructed based on the vectors pHANNIBAL and pART27.The work will lay the foundation for breeding a low morphine and high thebaine poppy.
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Received: 02 March 2009
Published: 07 December 2009
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