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| The Influence of α-synuclein Overexpression on Mitochondrial Membrane Structure with Atomic Force Microscopy |
| ZHAO Chun-li,ZHU Yuan-gang,DUAN Chun-li,LU Ling-ling,ZHANG Ling,YANG Hui |
| Beijing Institute for Neuroscience, Capital Medical University, Beijing Center of Neural Regeneration and Repair, Key Laboratory for Neurodegenerative Disease of the Ministry of Education, Beijing 100069,China |
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Abstract Objective:To identify the effect of α-synuclein overexpression on mitochondrial membrane structure with atomic force microscopy. Methods α-syn expression was mediated by AAV (adeno-associated viral vector) and Recombinant AAV/α-syn and AAV/LacZ viral particles were stereotaxically injected in the left side of rat substantia nigra (SN) for rat model of α-synuclein overexpression. Mitochondria were isolated from rats SN of Brain. Mitochondria were analysis with JC1 staining, atomic force microscopy and Western Blot. Results By 16 weeks post-infection of AAV-α-syn，the level of α-syn increased about 2 times in mitochondrial fraction with Western Blot and mitochondrial membrane potential (ΔΨ) decreased with JC1 staining. Furthermore, mitochondria swelling and porous like structure formed on the mitochondrial membrane with atomic force microscopy. Conclusion The data suggested that α-syn could accumulate in mitochondria, might form mitochondrial membrane pores and lead to ΔΨ decreases. α-syn might lead to mitochondrial dysfunction in Parkinson’s disease.
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Received: 12 January 2009
Published: 07 December 2009
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