Preparation and Identification of Recombinant Vasoactive Intestinal Peptide

China Biotechnology

2009, Vol. 29

Issue (11): 29-35 DOI:

Preparation and Identification of Recombinant Vasoactive Intestinal Peptide

XIE Shan-shan,YU Rong-jie,ZENG Le,LI Juan,WANG Jing-jing,HONG An

Bio-engineering Institute of Jinan University, Guangzhou 510632, China

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Abstract

In order to prepare the recombinant vasoactive intestinal peptide (VIP) using intein mediated rapid purification system, the cDNA encoding the recombinant VIP was designed and synthesized according to the preference of E.coli, and then was cloned into the expression vector PTWIN. The recombinant plasmid PTWIN-VIP was transformed into expression host E.coli strain ER2566.The fusion protein consisting of the recombinant VIP, intein and chitin binding domain was expressed and purified by chitin affinity chromatography. The target peptide was released from the fusion protein by changing the temperature and the pH of the cleavage buffer. The molecular weight of the recombinant VIP was determined by the mass spectrometry and the results was conformity with the theoretical value. The preliminary bioactivity assay indicated that the recombinant VIP decreased the serum resistin levels significantly in LPS-induced acute inflammation. The preparation and the characterization of antiinflammatory effects of the recombinant VIP layed the foundation for its further application.

Key words： Vasoactive intestinal peptide Intein Purification and identification Anti-inflammatory effects

Received: 18 July 2009 Published: 07 December 2009

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	XIE Shan-Shan
	TU Rong-Cha
	CENG Le
	LI Juan
	WANG Jing-Jing
	HONG An

Cite this article:

XIE Shan-Shan, TU Rong-Cha, CENG Le, LI Juan, WANG Jing-Jing, HONG An. Preparation and Identification of Recombinant Vasoactive Intestinal Peptide. China Biotechnology, 2009, 29(11): 29-35.

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https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2009/V29/I11/29


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