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| Construction of CMO-BADH Doublegene Expression Vector and Its Expression in Chinese wildrye |
| QU Tong-bao1,DENG Chuan2,WANG Pi-wu1
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1.Jilin Agricultural University, Changchun 130118, China
2.Jilin University,Changchun 130062,China |
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Abstract Chinese wildrye (Leymus chinensis) is an important grass of the Gramineae family,which widely grows in the natural grassland of Northern China. In order to protect the resource of Chinese wildrye and culture new variety, the gene of CMO and BADH being transfered to Chinese wildrye by particle accelerator. A doublegene plant expression vector，pCAMBIA-1301-CMO-BADH were reconstructed based on PC1301 plasmid by CMO and BADH which were both driven by 35S promoter; It was transferred to Chinese wildrye by genegun, detected by PCR analysis and Sorthern blotting respectively which conclude that the foreign gene has consistented with the recipient plants’ genome and expresses normally. The analysis of the content of betaine showed that it was higher in the doublegene vector transgenic plants than the blank plants.
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Received: 07 April 2009
Published: 07 December 2009
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Corresponding Authors:
QU TongBao
E-mail: peiwuw@yahoo.com.cn
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