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P300 Enhances Nanog Transcriptional Activity Through the Homeobox Domain |
1.State Key Laboratory of Biomembrane and Membrane Biotechnology,Institutes of Biomedicine,School of Medicine,Tsinghua University,Beijing 100084,China
2.Key Laboratory of Regenerative Biology,Guangzhou Institute of Biomedicine and Health,Chinese Academy of Sciences,Guangzhou 510663,China
3.National Engineering Laboratory for Tree Breeding,Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants,Ministry of Education,Beiming Forestry University,Beijing 100083,China. |
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Abstract Embryonic stem cells (ES cells) undergo selfrenewal to maintain their pluripotency. The selfrenewal and pluripotency of ES cells are under tight control of many transcription factors. Nanog is one of these transcription factors. However, the molecular mechanisms under which Nanog regulates ES cell pluripotency are not very clear. P300 is a general coactivator in eukaryotes which usually helps transcription factors to regulate downstream genes. Nanog (or Nanog mutants) and its reporter gene were cotransfeted with p300 in cells in order to see if p300 could affect Nanog transcriptional activity. The results showed that p300 enhances Nanog transcriptional activity through the homeobox domain.
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Received: 05 May 2009
Published: 29 October 2009
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Corresponding Authors:
Yun-Qian GUO
E-mail: pei_duanqing@gibh.ac.cn
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