Osmotic Press Plays an Important Role in Metabolic Control of <em>Saccharomyces ellipsoideus</em>

China Biotechnology

2011, Vol. 31

Issue (5): 75-80 DOI:

Osmotic Press Plays an Important Role in Metabolic Control of Saccharomyces ellipsoideus

XIE Tao, ZHANG Ru

Department of Chemical Engineering, Hunan Institute of Engineering, Xiangtan 411104, China

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Abstract

Using triose phosphate isomerase deficient (tpiΔ) mutant of Saccharomyces ellipsoideus as the control, the regulation of osmotic press on the balance between glycerol production and volatile acid formation was first studied. The results demonstrated that there existed the control of osmotolerance on the balance between dihydroxyacetone phosphate (DHAP) and glyceraldehyde-3-phosphate (GA3P) in wild S. ellipsoideus and the suitable high osmotolerance can transport metabolic flux from GA3P to DHAP to produce much glycerol. However, the control of osmotolerance didn't exist in tpiΔ mutant of S. ellipsoideus. In comparison with wild S. ellipsoideus, the tpiΔ mutant greatly reduced the yield of glycerol production and much created glycolytic metabolites because of GA3P accumulation at the same conditions. Therefore, the conclusion was made that, at the suitable high osmotolerance, DHAP accumulation of wild S. ellipsoideus would be helpful to regulate the ratio of glycerol to volatile acid.

Key words： Osmotolerance Saccharomyces ellipsoideus Metabolic regulation Triosephosphate isomerise(TPI)

Received: 19 October 2010 Published: 27 May 2011

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Cite this article:

XIE Tao, ZHANG Ru. Osmotic Press Plays an Important Role in Metabolic Control of Saccharomyces ellipsoideus. China Biotechnology, 2011, 31(5): 75-80.

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https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2011/V31/I5/75

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