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| The Development of a Modified and Simple Process for the Purification of Plasmid DNA |
| ZHANG Ping-jing, LI Zhong-ming, LIU Qing-liang |
| Shanghai H & Q Biotech Lid Cop, Shanghai 201203,China |
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Abstract A modified and simple manufacturing scale process for the purification of plasmid DNA was developed with low cost. The Escherichia coli JM108 host strain was selected for the fermentation with protein free media because of its advantage to produce predominantly super coiled plasmid DNA. The alkaline lyses method was used. The plasmid purification process including chromatography-absorbent silica, suitable DNA binding solution, endotoxin-removal reagents was substantially modified. The tests for the quality control were established for the detection of the residue impurities. The purity of the plasmid DNA obtained by this process can meet the clinical quality requirements.
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Received: 08 September 2010
Published: 26 April 2011
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