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中国生物工程杂志

China Biotechnology
China Biotechnology  2011, Vol. 31 Issue (04): 87-91    DOI:
    
Effect of Reinforcing Acetyl-CoA Supply in Docosahexaenoic Acid Production by Schizochytrium sp.
WEI Ping, REN Lu-jing, JI Xiao-jun, TONG Qian-qian, FENG Yun, HUANG He
College of Biotechnology and Pharmaceutical Engineering, State Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing University of Technology, Nanjing 210009, China
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Abstract  

In order to achieve efficient fatty acid accumulation in microorganisms,a continuous supply of acetyl-CoA directly in the cytosol of the cell should be satisfied. Considering that acetyl-CoA is the precursor in both the fatty acids synthetic and mevalonate pathway, the mevalonate pathway was inhibited and then more acetyl-CoA might be channeled to the fatty acids synthesis. A method of addition of precursor or/and the inhibitors of mevalonate pathway were used to reinforce acety-CoA supply. Acetate acid is the putative precursor of acetyl-CoA. Simvastatin and citrate acid are the inhibitors of mevalonate pathway. The effects of acetate acid, simvastatin and citrate acid on the performance of docosahexaenoic acid (DHA) production by Schizochytrium sp. were investigated. Including addition of acetate acid at the beginning or/and in the late period of fermentation, addition of simvastatin or citrate acid, the supplementation with acetate acid and simvastatin or citrate acid at the beginning of fermentation. The results showed that the highest of DHA yield reached 13.21g/L by combinations of 6mmol/L acetate acid and 1μmol/L citrate at the beginning of fermentation, which was 46.61% higher than that of control.



Key wordsAcetyl-CoA      Schizochytriumsp.      Docosahexaenoic acid     
Received: 08 November 2010      Published: 26 April 2011
ZTFLH:  Q819  
Cite this article:

WEI Ping, REN Lu-jing, JI Xiao-jun, TONG Qian-qian, FENG Yun, HUANG He. Effect of Reinforcing Acetyl-CoA Supply in Docosahexaenoic Acid Production by Schizochytrium sp.. China Biotechnology, 2011, 31(04): 87-91.

URL:

https://manu60.magtech.com.cn/biotech/     OR     https://manu60.magtech.com.cn/biotech/Y2011/V31/I04/87


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