Study on Superimposed Mutation on <em>Micromonospora purpurea</em> and Fermentation Process in 5L Fermentor

China Biotechnology

2011, Vol. 31

Issue (04): 77-82 DOI:

Study on Superimposed Mutation on Micromonospora purpurea and Fermentation Process in 5L Fermentor

GUAN Yu-xia, SUN Jia-xian, WANG Jing, ZHENG Zun-ji

College of Biological Science and Technology, Fuzhou University, Fuzhou 350002, China

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Abstract

Gentamicin producing strain Micromonospora purpurea was treated with 15μg/ml penicillin when M. purpurea had cultured 36h, and the cells that had cultured for 48h at logarithmic growth phase were treated with UV and NTG, then they were cultured in incubator at 37℃ for 5~8 days, and when the colonies grew out, the cells were treated by UV again. As a result, a high yield strain N-14 was obtained. Compared to control, the biological titer of N-14 fermented in tube was increased from 625u/ml to 1913u/ml, and the biological titer of N-14 fermented in flask was 2178u/ml and increased by 75.2% compared to control 1243(u/ml). Besides, the reducing sugar, total sugar, amino nitrogen, pH, cell concentration and other metabolic parameters were determined during fermentation process in 5L fermentor, and the culture condition was researched. The average biological titer of 5 batches was 1967u/ml and was increased by 68% compared to control 1171u/ml.

Key words： Micromonospora purpurea Superimposed mutation Biological titer Metabolic parameter

Received: 26 October 2010 Published: 26 April 2011

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Cite this article:

GUAN Yu-xia, SUN Jia-xian, WANG Jing, ZHENG Zun-ji. Study on Superimposed Mutation on Micromonospora purpurea and Fermentation Process in 5L Fermentor. China Biotechnology, 2011, 31(04): 77-82.

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https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2011/V31/I04/77

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