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| Development of a Freeliving Nematode Panagrellus redivivus in Saccharomyces cerevisiae with cip Genes |
| YOU Juan1, HUANG Jian-lin2, CAO Li3, HAN Ri-chou3 |
1. Department of Biochemistry & Molecular Biology, Guangdong Pharmaceutical University, Guangzhou 510006, China;
2. Guangzhou Institute of Metrology & Testing Technology, Guangzhou 510030, China;
3 Guangdong Entomological Institute, Guangzhou 510260, China |
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Abstract CipA and CipB are two types of intracellular crystalline inclusion proteins produced by Photorhabdus luminescens bacteria, which are symbionts of entomopathogeic Heterorhabditis nematodes. To understand the biological function of these proteins for free living Panagrellus redivivus nematodes, recombinant Saccharomyces cerevisiae expression system of Cip proteins were constructed and the resulting yeast cells were used to feed the sterile J1 juveniles of P.redivivus. In recombinant yeast cells with CipA and/or CipB, the nematodes developed about 24 h faster than those in the yeast cells without Cip proteins. This promotion was reflected in two aspects: to short the cycle time and to enhance the reproductive ability of P. redivivus nematode. It means that the nutrient sources from entomopathogeic nematodes are acceptable by this free-living nematode.
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Received: 16 August 2010
Published: 26 April 2011
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