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| STAT3 Silencing Inhibits Migration of Murine Breast Cancer Cells in vitro |
| XU Liang,GU Yu-chao,SHI Han,MI Wen-yi,YU Wen-gong |
| College of Medicine and Pharmaceutics Ocean University of China, Qingdao 266003,China |
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Abstract STAT3 (signal transducer and activator of transcription 3) is a dual functional transcription factor with functions as signal transduction and transcription regulation. There are reports that the expression of STAT3 in breast cancer is significantly higher and STAT3 can facilitate breast cancer metastasis. To clarify the definite effect and molecular mechanism of STAT3’s involvement in breast cancer metastasis, STAT3 was silenced by RNAi in the murine highly metastatic 4T1 cells. The silencing of STAT3 didn’t affect the proliferation rate of breast cancer 4T1 cells in MTT assay. However, the migration activity of STAT3 knockdown cells was strongly reduced in Transwell assay. The real-time quantitative PCR results indicated that STAT3 silencing significantly suppressed the expression of VEGF, IL-6 and mosin, while increased E-cadherin expression. Western Blot assay showed that phosphorylation level of MAPK was reduced in STAT3 knockdown cells. The results of this study demonstrate that STAT3 plays an important role in breast cancer metastasis and provide experimental evidences in therapy with STAT3 as a target.
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Received: 07 January 2010
Published: 12 June 2010
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