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A Chromotography Method for Removing Impurity Proteins from Meningococcal Polysaccharide Type A Sample |
XIE Hong-yan,WANG Ji-yuan,LIU Jing,WANG Yi-jun,ZHAO Zhan-yong,WU Yun-tao,MIAO Jin-yun,WU Yuan-yuan |
GE Healthcare Co.,Shanghai 201203,China |
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Abstract Objective:Set a new chromatography method to remove impurity proteins from meningococcal polysaccharide type A sample instead of the traditional phenol abstraction method. Method:This new method includes two purification steps by using Capto adhere and Sephardex G-25. Result:The contaminative protein concentration is 0.2% in final meningococcal polysaccharise type A sample, which is below the WHO regulation standard (1%),the content of nucleic acid and endotoxin are also blow the current regulation standard,the recovery is over 65%. Conclusion:Compare with the tradition phenol abstraction method, the chromatography method can remove impurity proteins effectively with high recovery and reducing environment pollution and easy to scale up.
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Received: 25 June 2010
Published: 25 August 2010
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