Optimization of Autotrophic Cultivation of Lipids Production for Biodiesel by Chlorella vulgaris with Response Surface Methodology

doi:Q819

China Biotechnology

2010, Vol. 30

Issue (08): 106-111 DOI: Q819

Optimization of Autotrophic Cultivation of Lipids Production for Biodiesel by Chlorella vulgaris with Response Surface Methodology

ZHENG Hong-li¹,GAO Zhen¹,HUANG He^1,2,JI Xiao-jun¹,BAI Yue-hua³,LI Wen-qi³

1.College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210009, China
2.State Key Laboratory of MaterialsOriented Chemical Engineering, Nanjing 210009, China
3.Petroleum Research Institute, China National Petroleum Corporation, Beijing 100029, China

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Abstract

Response surface methodology was used to optimize the autotrophic cultivation conditions for lipids production by Chlorella vulgaris in a 2-L airlift photobioreactor. In the first optimization step, a Plackett-Burman design was used to evaluate the influence of ten related factors and it was found out that KNO3 concentration, temperature and CO2 concentration influenced lipids production significantly. Subsequently, the path of steepest ascent was used to approach the optimal region of the cultivation conditions. In the third step, KNO3 concentration, temperature and CO2 concentration were further optimized using central composite designs and response surface analysis and the optimum conditions were that KN03 concentration was 0.31 g/L, temperature was 26.5 ℃ and CO2 concentration was 6.80%. Under optimum conditions, the lipid yield was 0.42 g/L and it was increased by two times than that under the conditions before optimized. Chlorella vulgaris was cultured in a 10-L airlift photobioreactor under optimum conditions.

Key words： Chlorella vulgaris Cultivation conditions Response surface Methodology BiodieselLipids

Received: 21 April 2010 Published: 25 August 2010

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	ZHENG Hong-Li
	GAO Zhen
	HUANG He
	JI Xiao-Dun
	BAI Ti-Hua
	LI Wen-Qi

Cite this article:

ZHENG Hong-Li, GAO Zhen, HUANG He, JI Xiao-Dun, BAI Ti-Hua, LI Wen-Qi. Optimization of Autotrophic Cultivation of Lipids Production for Biodiesel by Chlorella vulgaris with Response Surface Methodology. China Biotechnology, 2010, 30(08): 106-111.

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https://manu60.magtech.com.cn/biotech/Q819 OR https://manu60.magtech.com.cn/biotech/Y2010/V30/I08/106

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