中国生物工程杂志

Abstract: This study was designed to investigate the usefulness of urinary nucleosides in the diagnosis of primary hepatic carcinoma. By using micellar electrokinetic capillary chromatography (MECC )method , urinary nucleosides were determined in an uncoated 50cm×75μm i. d capillary tube using a optimized 250 mmol•L-1SDS–20 mmol•L-1 sodium tetraborate–40 mmol•L-1 sodium dihydrogenphosphate buffer（pH=6.8）, 10kV voltage, 2psi×s injection, 25℃ temperature and UV detection at 260nm. The nucleosides were extracted from urine by phenylboronate affinity gel chromatography. Ten kinds of normal and modified nucleosides were determined in urine samples from 20 healthy individuals and 20 patients of primary hepatic carcinoma, it was showed that the contents of urinary nucleosides excretion, especially modified nucleoside in patients of primary hepatic carcinoma were higher than those in normal healthy persons. These results provided a kind of probably means for the assessindividualst of the urinary nucleosides in cancer clinical diagnoses.