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中国生物工程杂志

China Biotechnology
China Biotechnology  2009, Vol. 29 Issue (08): 119-123    DOI:
    
Preparation of DNA Molecular Weight Standards: Methods and Advances
 ZHANG Ying1, LIU Fa-Zhen1, LI Qiang1, YE Chun-Jiang1,2
1.Department of Bioscience and Biotechnology, College of Medicine and Life Sciences, University of Jinan, Jinan250022, China
2.State Key Laboratory of Microbial Technology, College of Life Science, Shandong University, Jinan250100, China
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Abstract  

DNA molecular weight standards are a mixture of a grou Pof DNA molecules with known sizes to reference the molecular weight (mass) of unknown DNA samples in nucleic acid electrophoresis and hel Pthe experimentalists to judge the property of the DNA samples. At the present time the DNA molecular weight standards are essential for nucleic acid electrophoresis analysis in the molecular biology and genetic engineering fields. The methods and principles for the production of DNA molecular weight standards with the recent advances in this field were summarized.



Key wordsDNA molecular weight standards      Vector construction      PCR technology      Restrictive digestion     
Received: 03 April 2009      Published: 28 July 2009
ZTFLH:  Q819  
Fund:  

Ph.D Fellow Fund of Univerity of Jinan;Ph.D Fund fo Shandong Province

Corresponding Authors: Chunjiang Ye   
Cite this article:

ZHANG Ying, LIU Fa-Zhen, LI Jiang, XIE Chun-Jiang. Preparation of DNA Molecular Weight Standards: Methods and Advances. China Biotechnology, 2009, 29(08): 119-123.

URL:

https://manu60.magtech.com.cn/biotech/     OR     https://manu60.magtech.com.cn/biotech/Y2009/V29/I08/119

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