| 研究报告 |
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| Investigate antibiosis dynamics and antibacterial mechanisms of Antibacterial Peptides from Musca domestica Larvae |
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Abstract The antibacterial peptides from Musca domestica larvae that were induced by injection with Salmonella typhimurium. Antibiosis dynamics of this antibacterial peptides was investigated. And the antibacterial mechanisms was analyzed. There were one or two activity peaks in 24 hours of the antibacterial peptides sample. The same antibacterial peptides sample had different antibiosis dynamics property for different bacteria. The action time and mode of Musca domestica larvae antibacterial peptides were decided by the characteristic of bacteria cell. The results of scanning electron microscope(SEM) , cell phosphorus metabolism essay, cell ultraviolet absorbate detection and the interaction of antibacterial peptides and cell’s DNA essay showed that the antibacterial peptides of Musca domestica larvae induced by microbe not only effected on the cellular membrane but also influenced the intern structure and function of bacterial cell and binded with bacterial DNA. The interpretation of antibiosis dynamics is that one kind of antibacterial peptides made cellular membrane ruptured caused the first activity peak of antibiosis dynamics, the other kind of antibacterial peptides entered bacterial cell and reacted with genetic material caused the second activity peak.
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Received: 16 May 2006
Published: 25 November 2006
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