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Lipase Extraction of Cryytococcus neoformans by Aqueous Two-phase System |
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Abstract Lipase extraction of Cryytococcus neoformans using aqueous two-phase system has been studied in this paper.The polyethylene glycol(PEG)/salt system has been systematicly researched,the system of PEG/(NH4)2SO4 was better to separate this lipase.Many factors which affecting the ability of phase separation and extraction has been investigated. In this case, PEG 15%,(NH4)2SO4 22.5%, pH8.0 without NaCl was the optimized condition,and the partition coefficient and purification multiple was 6.8 and 7.5 respectively.Its specific activity got to 40.3 U/mg protein.
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Received: 24 October 2006
Published: 25 June 2007
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