Abstract In this paper the influence of cell preparation methods of Ralstonia solanacearum on the cell viability and cell surface properties was studied. The results showed that the batter method was as follows: bacterial cultures harvested by centrifugation at 5000×g for 10 min, washed and centrigugated twice, resuspended in millipore water (＞16MΩ) ). Using this method could not only avoid the interference of medium, but also keep the cell viability and cell surface properties.
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