Cloning and expression of two MADS-box genes from Castanea mollissima

China Biotechnology

2006, Vol. 26

Issue (0): 76-78 DOI:

Cloning and expression of two MADS-box genes from Castanea mollissima

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Abstract

Abstract: we cloned two completely new MADS-box genes from Castanea mollissima by the method of 3’RACE, named CmMADS2(DQ148294) and CmMADS4(DQ148296) respectively. CmMADS2 encoded 242 identical amino acids (aa) including 56 aa of the MADS-box domain and CmMADS4 encoded 242 identical amino acids (aa) including 72 aa of the MADS-box domain. RT-PCR analysis showed that CmMADS2 expressed in inflorescences and tender leaves with flowers, not in other tissue(fruit﹑old leaves﹑stem and tender leaves with no flowers). While CmMADS4 was expressed in all the tissue mentioned above, suggesting that these novel MADS-box genes may be involved in floral organ development of Castanea mollissima.

Key words： RT-PCR MADS-box Castanea mollissima

Received: 07 March 2006 Published: 15 June 2006

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. Cloning and expression of two MADS-box genes from Castanea mollissima. China Biotechnology, 2006, 26(0): 76-78.

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https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2006/V26/I0/76

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