研究报告 |
|
|
|
|
Fetal membrane derived adherent cells:a novel source for mesenchymal stem cells |
|
|
|
|
Abstract Objective: To established a method for isolation and purification of fetal membrane derived adherent cells (FMDACs), and investigate the biological characteristics of FMDACs. Method: FMDACs were isolated with trypsin and cultured in vitro. Inducing FMDACs in vitro differentiate into osteoblasts and adipocytes. FACS and immunocytochemistry technique was used to examine the cell surface antigen. The genetic stability was verified by karyotype analysis. Results: Fetal membrane derived adherent cells were successfully isolated and expanded in vitro. It had strong proliferative ability. FMDACs were positive for CD44 and CD29, but negative for CD34, CD14 and CD45. FMDACs were differentiated into osteoblasts and adipocytes after inducement. The karyotype was stable in the sixth-passaged FMDACs and the tumorigenicity was not found. Conclusion: FMDACs have the possibility of a multipotent stem cells, which have strong capacities of self-renewal and multidirectional differentiation. The genetic background of FMDACs was stable. FMDACs may be used as a kind of novel seed cells for tissue engineering.
|
Received: 28 December 2005
Published: 25 July 2006
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
|
Shared |
|
|
|
|
|
Discussed |
|
|
|
|