Abstract In vitro culture of spermatogonial stem cells is a basic technology of biology ,which can promote the biology investigation of spermatogonial stem cells . So spermatogonial stem cells in vitro culture has become one of the hot fields in the Biology of the Stem Cell in recent years .Obtaining SSCs with high purity , plenty of vigour ,biological safty are the essential steps for SSCs related biological studies in vitro . However ,there are many factors affecting SSCs in vitro culture , such as animal species and age , culture medium , serum , selection of feeder layer and growth factors . SSCs always be thought not to culture in a long-term time in vitro ,which retain stem cell activity . But with the deeper and deeper investigation , people have already obtained the long-time culture of SSCs , specially in Murine . Murine is a direct investigation analogue organism to research mammalian even human in the end .This review mainly summarize the factors ,which affect SSCs in a long-term culture in vitro according to the recent related reports and our experimental work
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