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The Assembly of a Novel Enzyme Biosensor for Aflatoxin B1 Detection |
Shen Yi YAO Dong-sheng YAO |
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Abstract A novel biosensor for aflatoxin B1 detecting has been reported in this paper. The biosensor electrode for AFB1 detecting was assembled by immobilized aflatoxin-oxidoreductase using open-ended multi-walled carbon nanotubes as matrix. Its linear range was between 0.16μM and 3.2μM. And if the specific anti-aflatoxin B1 antibody and aflatoxin oxidoreductase were both immobilized on the electrode with Multi-Walled carbon nanotubes, the detection limit of the modified electrode could be 16 nM with a 10 times improved sensitivity. The aflatoxin enzyme biosensor assembled this way strode one step forward its practical application.
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Received: 16 November 2007
Published: 28 March 2008
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Corresponding Authors:
YAO Dong-sheng YAO
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