A Method of Construction Polycistron Tandem Gene of Small Peptide

China Biotechnology

研究报告

A Method of Construction Polycistron Tandem Gene of Small Peptide

Download:

HTML

PDF(0KB) HTML
Export: BibTeX | EndNote (RIS)

Abstract Objective: To construct a polycistron tandem repeated Echistatin (Ecs) gene. Methods: Three Ecs genes with independent initiation and termination codon were ligated tandem through restriction enzyme sites after amplified with 3 pairs of primers using pMD18T-Ecs as template. The polycistron Ecs gene was inserted into pET30a and expressed in E.coli BL21(DE3) with IPTG induction. The expression results were identified by 18% SDS-PAGE and Western blot. Results: The expression of Ecs polycistron was accomplished with 18% expression level of total protein determined by SDS-PAGE and Western blot. Conclusion: The successful expression of Ecs polycistron provides a new method for the preparation of low molecular weight protein.

Key words： Cloning and expression E.coli Echistatin Polycistron

Received: 11 July 2006 Published: 25 November 2006

	Service
	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors

Cite this article:

. A Method of Construction Polycistron Tandem Gene of Small Peptide. China Biotechnology, 2006, 26(11): 45-47.

URL:

https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2006/V26/I11/45

[1]	TONG Mei,CHENG Yong-qing,LIU Jin-yi,XU Chen. Construction of a Strain for Promoting Production of Small Molecule Antibodies in Periplasmic Space of Escherichia coli[J]. China Biotechnology, 2020, 40(5): 48-56.

[2]	YANG Li,SHI Xiao-yu,LI Wen-lei,LI Jian,XU Han-mei. Optimization of Electroporation Conditions in Construction of Phage Display Antibody Library[J]. China Biotechnology, 2020, 40(4): 42-48.

[3]	Hang Hai-ying,Liu Chun-chun,Ren Dan-dan. Development, Application and Prospection of Flow Cytometry[J]. China Biotechnology, 2019, 39(9): 68-83.

[4]	ZHANG Yu-meng, TONG Mei, LU Xiao-dong, MI Yue, MO Ting, LIU Jin-yi, YAO Wen-bing. *Expression of Soluble Anti-TNF-α Fab in E.coli: Optimization for Technological Process*[J]. China Biotechnology, 2016, 36(9): 31-37.

[5]	ZHANG Yu-meng, TONG Mei, LU Xiao-dong, MI Yue, XU Chen, YAO Wen-bing. Advances in Promoting Soluble Expression of Recombinant Protein in Escherichia coli[J]. China Biotechnology, 2016, 36(5): 118-124.

[6]	ZHENG Li-juan, CHEN Shao-yun, XU Gang, WU Jian-ping, YANG Li-rong. *Engineering E.coli* for Isobutanol Production by Two-promoter Vectors**[J]. China Biotechnology, 2013, 33(8): 67-74.

[7]	LUO Feng, DUAN Xu-guo, SU Ling-qia, WU Jing. Cloning,Expression and Fermentation Optimization of Thermobifida fusca Trehalose Synthase Gene in E.coli[J]. China Biotechnology, 2013, 33(8): 98-104.

[8]	GAO Ting-ting, ZHANG Qi, WEI Yun-lin, JI Xiu-ling, LIN Lian-bing. *Cloning and Expression of the dCTP Deaminase Gene from Thermus* Phage TSP4**[J]. China Biotechnology, 2013, 33(4): 34-39.

[9]	LIU Qi-gang, DAI Yun-jian, ZHANG Yong-xia, WANG Bao-cheng, WANG Ming-rong. *Efficient Soluble Expression of Anti-IgE scFv in E.coli* and Optimization of Expression Conditions**[J]. China Biotechnology, 2012, 32(11): 23-28.

[10]	LIU Qi-gang, DAI Yun-jian, ZHANG Yong-xia, WANG Bao-cheng, WANG Ming-rong. *Efficient Soluble Expression of Anti-IgE scFv in E.coli* and Optimization of Expression Conditions**[J]. China Biotechnology, 2012, 32(11): 23-28.

[11]	ZHAN Sheng, YIN Xing-feng, LI Hui, LI Nan, YANG Xiao-yan, SUN Xue-song. Effect of the Incubation Time in the Enrichment of the Phosphopeptides with Titanium Dioxide[J]. China Biotechnology, 2012, 32(03): 59-62.

[12]	FU Feng-gen, XU Zheng, LI Gui-xiang, LI Sha, FENG Xiao-hai, XU Hong. *D-tagatose Production Utilizing Immobilized Recombinant Escherichia coli* cells**[J]. China Biotechnology, 2011, 31(7): 85-90.

[13]	NIU, Wei-ning, YANG Meng-lin, CAO Shan-shan, XU Le, QIN Chuan-guang. Expression, Purification and Activity Assay of the Full-length and Truncated Human Cystathionine β-Synthase[J]. China Biotechnology, 2011, 31(12): 15-21.

[14]	LI Rui-fang, XIONG Qian-cheng, ZHANG Zong-wu, HUANG Liang, WANG Bin. Construction and Identification of Polycistronic Expression Plasmids of Antifungal Peptide CGA-N46 Gene[J]. China Biotechnology, 2011, 31(12): 93-98.

[15]	ZHU Lei, WANG Qing-min, WU Guo-dong, XUE Tong-tong, SUN Li-xia, WANG Jing-yi. Expression, Purification and Activity Identification of Urate Oxidase in Escherichia coli[J]. China Biotechnology, 2011, 31(04): 83-86.

Viewed

Full text

Abstract

Cited

Shared

Discussed