Study on Embryonic Stem Cell from Cultured Primordial Germ Cells of

China Biotechnology

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Study on Embryonic Stem Cell from Cultured Primordial Germ Cells of

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Abstract The primitive germ cell is a kind of new cell resources used as isolation and coloning embryonic stem (ES)cell .In this study,genital ridge and the surrouding tissue from 30~45 day old fetuses of Mongolia sheep were minced with the machine and disaggregated by trypsin/EDTA solution,and then ,PGCS and it’s fetal fibroblast were co-cultured at 38℃,5%CO2 concentration and saturated humidity in a CO2 incubator. Dublecco’s Modified Eagle’s Medium(DMEM)with 10% fetal bovine serm (FBS) was used as culture medium without any cell growth factors.

Key words： Cell culture Mongolia sheep Primitive germ cell(PGC s) Embryonic stem (ES) like cell

Received: 06 July 2006 Published: 25 November 2006

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. Study on Embryonic Stem Cell from Cultured Primordial Germ Cells of. China Biotechnology, 2006, 26(11): 40-44.

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https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2006/V26/I11/40

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