研究报告 |
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The investigation of the effect on human Hepatoma Cell apoptosis induced by Resibufogenin |
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Abstract AIM to study the mechanism of Resibufogenin inhibited on the growth of human hepatoma cell by analyzing the apoptosis induced by Resibufogenin. METHODS The apoptosis was observed by means of Flow Cytometry. The effects of Resibufogenin on DNA synthesis and expression of correlative proteins in hepatoma cell apoptosis was observed by means of Western blotting and cytochemical method. RESULT It was showed that Resibufogenin could induce Bel7402 cell (human hepatoma cell) to apoptosis. The rate of apoptosis exceeds 50 percent. The expression of inhibiting apoptosis gene bcl-2 decrease after 24 hours in the group of 1.0 μm Resibufogenin and decreased more significantly after 48、72 hours; the expression of promoting apoptosis gene Bax increased after 24 hours and increased more significantly after 48、72 hours. CONCLUSION the resμlt indicate that inducing neoplasm cell apoptosis may be one mechanism of the Resibufogenin inhibiting and killing the human Hepatoma Cell .
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Received: 24 October 2005
Published: 25 June 2006
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