Cloning, Expression the Recombinant AtPCS1 and Preparation

China Biotechnology

2006, Vol. 26

Issue (0): 139-142 DOI:

Cloning, Expression the Recombinant AtPCS1 and Preparation

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Abstract

The full length of AtPCS1 gene was amplified by RT-PCR. After sequencing, the gene was cloned into the expression vector pET28a to contrast AtPCS1 expression plasmid pET28a-AtPCS1. The E.coli BL21 contained the expression plasmid was induced by 0.75mM IPTG and the protein was purified using SDS buffer. Mouse was immunized with the purified protein. The results of ELISA showed that the titer of the anti-serum was about 1:2000 and the western-blot analysis of the anti-serum with purified protein AtPCS1-His or the total protein of Arabidopsis thaliana show the polyclonal anti-AtPCS1-His serum has high activity and speciality.

Key words： anti-serum phytochelatin synthase prokaryotic expression

Received: 10 January 2006 Published: 15 June 2006

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. Cloning, Expression the Recombinant AtPCS1 and Preparation. China Biotechnology, 2006, 26(0): 139-142.

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https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2006/V26/I0/139

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