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The application of NICE system in microbiology |
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Abstract The NICE system had been abroad used in Microbiology research ever since Kuipers et al. first brought it forward early in 1995, now this system gained greater promising application and commercial foreground especially when it was used in medical research recently. This mini-review is provided of the different applications in lactococci and other Gram-positive bacteria: (1) over expression of homologous and heterologous genes, (2) metabolic engineering,(3) expression of prokaryotic and eukaryotic membrane proteins, (4)protein secretion and anchoring in the cell envelope, (5) expression of genes with toxic products and analysis of essential genes.
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Received: 15 December 2005
Published: 15 June 2006
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