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| Fermentation Characteristics Research of Recombinant Escherichia coli for Succinate Production |
| YU Li,MA Jiang-feng,YUE Fang-fang,LIU Shu-wen,JIANG Min |
| State Key Laboratory of MaterialsOriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210009, China |
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Abstract The fermentation characteristics of recombinant E.coli JM001(△ppc)/pTrc99apck for succinate production was investigated. The results showed that its glucose consumption and succinate production rates were 4.2 and 15.3 folds as much as those of the parent strain E.coli JM001, respectively. The mass yield of succinate to glucose was 54%, besides the acetic acid concentration was rather low. It could be concluded that the reaction from phosphoenolpyruvate (PEP) to oxaloacetic acid (OAA) catalyzed by PEP carboxykinase (PCK) was more beneficial to the cell growth and succinate production compared to PEP carboxylase (PPC). Further investigation was carried out to optimize the fermentation conditions. The results showed that bacterial sludge was much better than 10% (v/v) broth for the inoculation from aerobic phase to the anaerobic one, succinate mass yield increased from 55.2% to 65.1% and the entire time to consume 20g/L glucose reduced to 48h. The results of glucose tolerance experiment showed that between 20g/L and 60g/L initial glucose, cell growth and succinate production were both satisfactory, succinate mass yield was from 64.18% to 69.10% with the increase of glucose concentration. But when the glucose was higher than 60g/L, cell growth and succinate production were clearly repressed. The higher the glucose concentration was, the more obvious the inhibition effects were. For 7L fermentor research, the initial glucose of the anaerobic phase was 40.25g/L, after 96 h it was completely consumed. The succinate mass yield was 67.75%. During the anaerobic phase, the glucose consumption rate was 0.42g/(L·h) and succinate productivity was 0.28g/(L·h).
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Received: 14 April 2010
Published: 25 August 2010
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