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Development of Chimera Protein: sFcεRIα/mIg(IgG2) |
MA Jin-wei, CHENG Hong, CHU Xue-zhe, LI Xian-zhong, HU Yan-hong, YU Zhi-ai, LIN Feng, HE Li-hua, BAI Xian-hong, HU Pin-liang |
Biotech Pharmaceutical Ltd. Beijing 100176,China |
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Abstract IgE plays a key role in type I hypersensitivity reactions. The blocks of IgE binding to its high affinity receptor FcεRI can efficiently eliminate allergic reaction. The sFcεRIα/mIg(IgG2)was constructed by gene fusion of the extracellular portion of α subunit of FcεRI receptor with human IgG2 hing region, CH2 and CH3 region. The chimera could be a markedly longer plasma half-life than the soluble extracellular domain. This chimeric protein was produced by transfecting Chinese Hamster Ovary cells (CHO). Following affinity purification, the apparent molecular weight of sFcεRIα/mIg(IgG2), which showed high affinity with human and mouse IgE, was above 170 kDa. A good foundation for industrialization of this new engineered soluble protein for type I hypersensitivity diseases was provided.
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Received: 29 September 2009
Published: 25 October 2010
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Corresponding Authors:
HU Pin-liang
E-mail: 327hu@sina.com
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Cite this article:
MA Jin-wei, CHENG Hong, CHU Xue-zhe, LI Xian-zhong, HU Yan-hong, YU Zhi-ai, LIN Feng, HE Li-hua, BAI Xian-hong, HU Pin-liang. Development of Chimera Protein: sFcεRIα/mIg(IgG2). China Biotechnology, 2010, 30(10): 17-21.
URL:
https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2010/V30/I10/17
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