|
|
Study on Liquid Fermentation Conditions for Anthraquinone Pigment by Fusarium Oxysporum |
CUI Tang-bing,LIU Qing-xiang |
School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006,China |
|
|
Abstract The liquid fermentation conditions for anthraquinone pigment by Fusarium oxysporum was optimized. Single factor experiment and orthogonal optimization experiment determined the optimal fermentation conditions for production as follows: the optimate composition of the medium:soluble starch 30%,(NH4)2SO4 3%, MgSO4 0.3%, KH2PO4 4%,and the optimal fermentation conditions: initial medium pH 6.0, medium volume 20%, inoculation amount 10%, Tween -80 1.0%, incubation temperature 28℃, rotation speed 200 rpm and the fermentation period was 120h. In the optimal conditions, the color value reached 8.184U/ml, and increased by about 1.8 folds compared with the unoptimized conditions. It is the first time to study the anthraquinone pigment produced by Fusarium oxysporum at domestic. And it is hoped to lay the foundation for its further application.
|
Received: 13 April 2010
Published: 25 August 2010
|
|
|
[1] 郭承育.天然食用色素的开发应用.青海大学学报(自然科学版),2001,19(3):4547. Guo C Y. Journal of Qinghai University, 2001,19(3):4547.
[2] Eizo T,Timothy H M,Toshiro W,et al. Preventive effects of anthraquinone food pigments on the DNA damage induced by carcinogens in Drosophila . Mutation Research ,2001,480481:139145.
[3] 曹亮,周建军.蒽醌类化合物的研究进展.西北药学杂志,2009,24(3):237238. Cao L, Zhou J J. The Northwest Pharmaceutical Journal, 2009,24(3):237238.
[4] 徐任生.天然产物化学.第二版.北京:科学出版社,1993,12:578580. Xu R S.Natural Product Chemistry.2nd. Beijing: Science Press,1993, 12:578580.
[5] 李芳,史怀,刘波,等.淡紫拟青霉对尖抱镰刀菌的拮抗作用与机制分析.植物保护学报,2005,32(14):373378. Li F,Hi H,Liu B,et al. Acta Phytophylacica Sinica. 2005,32(14):373378.
[6] 刘新月,李凡,陈海如,等.致病性尖孢镰刀菌生物防治研究进展.云南大学学报,2008,30(S1): 8993. Liu X Y, Li F, Chen H R,et al. Journal of Yunnan University,2008, 30 (S1): 8993.
[7] Fravel D R, Deahl K L, Stommel J R.Compatibility of the biocontrol fungus Fusarium oxysporum strain CS20 with selected fungicides. Biological Control,2005,34:165169.
[8] Robert A B,James H T.Novel anthraquinones from stationary cultures of Fusarium oxysporum. Journal of Fermentation and Bioengineering,1998,85 (4):359361.
[9] 刘森,杨涛,崔薇薇.尖孢镰刀菌发酵生产木糖醇的工艺研究.现代食品科技,2008,24(4):347348. Liu S, Yang T, Cui W W. Modern Food Science and Technology,2008,24(4):347348.
[10] Tatiana F P, Gabriela A M.Optimizing the production of cutinase by Fusarium oxysporum using response surface methodology. Enzyme and Microbial Technology, 2007,41:613619.
[11] Charilaos X, Evangelos T, Petros K,et al. Evaluation of Fusarium oxysporum as an enzyme factory for the hydrolysis of brewer’s spent grain with improved biodegradability for ethanol production. Industrial Crops and Products,2008,28:213224.
[12] Rodrigo J S J,Benedict C O,Fatima M B,et al.Microbial consortium bioaugmentation of a polycyclic aromatic hydrocarbons contaminated soil. Bioresource Technology, 2008, 99: 26372643.
[13] 曹亮,周建军.蒽醌类化合物的研究进展.西北药学杂志,2009,24(3):237238. Cao L, Zhou J J. The Northwest Pharmaceutical Journal, 2009,24(3):237238.
[14] 楼志华,陶冠军,蔡宇杰,等.竹黄菌发酵天然色素及其结构的初步研究.天然产物研究与开发,2006,18:449452. Lou Z H,Tao G J,Cai Y J,et al. Nat Prod Res Dev,2006,18:449452.
[15] 管国强,张玲,黄达明,等.桑黄深层发酵上清液抗氧化活性的研究.食品工业科技, 2008,12:106107,192. Guan G Q,Zhang L,Huang D M,et al. Science and Technology of Food Industry, 2008,12:106107,192.
[16] 崔洪霞.胶州湾海洋链霉菌抑菌活性筛选、2株抑菌活性菌株的鉴定及次级代谢产物的研究. 中国科学院研究生院(海洋研究所),2004. Cui H X.Screening on Activity Marine Streptomyces in JiaoZhou Bay,Classification on Two Activity Strains and Studied on Metobalites. Chinese Academy of Sciences(Institute of Oceanology),2004.
[17] Nagia F A, ELMohamedy R S R.Dyeing of wool with natural anthraquinone dyes from Fusarium oxysporum. Dyes and Pigments . 2007,75:550555.
[18] 龙正海,严小军,张煜炯,等.一株分离自竹黄产蒽醌类色素菌株的鉴定及其培养特性.中南林业科技大学学报,2009,29(6): 8892. Long Z H,Yan X J,Zhang Y J,et al. Journal of Central South University of Forestry& Technology.2009,29(6):8892. |
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
|
Shared |
|
|
|
|
|
Discussed |
|
|
|
|