|
|
Cloning and Preliminary Analysis of the Stem Specific Promoter of Sugarcane |
CA Wen-Wei, WANG Zheng-Feng, ZHANG Shu-Zhen, YANG Ben-Feng |
Institute of Tropical Bioscience and Biotechnology|Chinese Academy of Tropical Agricultural Sciences| Haikou571101,China |
|
|
Abstract Sugarcane stem is an ideal organ for producing foreign pharmaceutical proteins and chemicals by genetic engineering. A perfect promoter driving foreign gene to express strongly and specifically in sugarcane stem is necessary for this purpose. In order to isolate a Sugarcane stemspecific promoter , a fragment of 1968bp nucleotide sequence(Ppst2a)upstream 5′ of sugarcane pst2a gene, which was demonstrated to express specifically in sugarcane stem previously was isolated by using chromosomal walking. Bioinformatical analysis of this sequence shows that the sequence contains some typical elements of a promoter. To identify the stemspecific of this promoter, a construct was derived from pCAMBIA1301, which original CaMV 35S promoter was replaced by the 1968bp nucleotide sequence, and named as pCAMBIA1900. Transformations of pCAMBIA1900 and pCAMBIA1301 to leaves and stem pieces of sugarcane were carried out by using particle bombardment. The transient expression of gus showed that the gus expressed specifically in sugarcane with a little higher level compared with CaMV 35S. It is the first report that pst2a promoter is a potential stemspecific promoter which can further be used in transgenes into sugarcane.
|
Received: 04 January 2009
Published: 28 July 2009
|
|
|
|
[1] McQualter R B, Chong F, Meyer K, et al. Initial evaluation of sugarcane as a production platform for phydroxybenzoicacid. Plant Biotechnology Journal, 2005, 3 (1):29~41
[2] Wang M L, Goldstein C, Winston S U, et al. Production of biologically active GMCSF in sugarcane: a secure biofactory. Transgenic Research, 2005, 14(2):167~178
[3] Casu R E, Grof C P, Rae A L, et al. Identification of a novel sugar transporter homologue strongly expressed in maturing stem vascular tissues of sugarcane by expressed sequence tag and microarray analysis. Plant Mol Biol, 2003, 52:371~386
[4] 姚伟,耿广良,余爱丽,等. 一种改良的转基因甘蔗基因组DNA提取方法.热带亚热带植物学报,2004,12(3): 257~260
Yao W,Gen G L,Yu A L,et al.An improved method for isolation of transyenic sugaarcane genomic DNA.Journal of Tropical and Subtropical Botany,2004,12(3):257~260
[5] Robimson D J. 1996, Environmental risk assessment of release of transgenic plants containing virusderived inserts. Transgen Res, 1996, 5:359~362 |
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
|
Shared |
|
|
|
|
|
Discussed |
|
|
|
|