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Advances on Mammalian Cell Expression System |
LI Guo-kun1, GAO Xiang-dong1, XU Chen2 |
1. School of Science & Technology, China Pharmaceutical University, Nanjing 210009, China; 2. Beijing Tri-prime Genetic Engineering Co. Ltd., Beijing 102600, China |
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Abstract Mammalian cells have become the preferred host cells for the manufacture of a wide range of biopharmaceuticals. Recombination proteins expressed by mammalian cell expression system approximate human forms due to post translation modification, and mammalian cell expression system is used widely to manufacture therapeutic recombinant proteins. The establishment of efficient expression system was paid close attention to by more researchers. Along with the development of genomics, transcriptomics, proteomics and metabolomics, great progress has been made in optimization of a mammalian expression system in recent years. Development of mammalian cell expression system through the construction of efficient expression vector, host cell transformation, high-throughput screening, and medium optimization were introduced.
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Received: 06 November 2013
Published: 25 January 2014
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